proximal region meaning in English
近侧区
Examples
- Trka , the high affinity ngf receptor , contains an intracellular domain with tyrosine kinase activity and an extracellular domain with three tandem leucine - rich motifs flanked by two cysteine clusters in their amino termini and two immunoglobulin - like domains in the more membrane - proximal region
膜外域含有8个亮氨酸的重复区域，其前后分别串联着一组半胱氨酸残基，紧挨细胞膜的是两个c2 -型免疫球蛋白样区ig和ig ；膜内域为酪氨酸激酶催化活性区。 - The predicated amino acid sequences contain two conserved functional domains : a dna - binding domain ( hmg - box ) and a transactivation domain . the conserved motif of group b homology lies immediate adjacent to c - proximal region of the hmg - box . similar structure and identity of sox2 gene among mammals , birds , amphibians and reptiles suggest that sox2 gene have evolutionary conserved roles
进一步分析表明，已巴滩2编码的238个氨基酸多肤链含有两个保守的功能区域:位于n端的hmg盒区( 1一78aa )和位于c端的转录激活区( 131一236aa ) ，以及一个保守的特征性b亚族同源区域( 79一90aa )与hmg盒区的c端相连。 - By yeast two - hybrid assay , aes was found to interact with gp130 intracellular region through its conserved q domain . results from the yeast two - hybrid assay , gluthione s - transferase fusion protein pull - down assay and immuno - co - precipitation assay indicated that the q domain of tle1 is capable of binding gp130 intracellular domain , and the intracellular membrane proximal region of gp130 containing conserved boxl and box2 motifs seemed essential for this interaction . to investigate the consequence of this interaction , tle1 - gfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with gp130 expression vector
在通过酵母双杂交分析确定aes通过q结构域与sp130分子胞浆区结合的基础上，为确定tle1分子是否也能通过保守的q结构域与gp130分子胞浆区结合，我们通过pcr扩增编码gp130胞浆区与tleq分子不同结构域的cdna ，构建了含有这些不同结构域的酵母双杂交载体，通过酵母双杂交分析证实： tle1分子通过其氨基端的q结构域与gp130分子胞浆区近膜段结合。